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Mrs CLERGE Adeline
Post doc

12/05/1991

02 35 52 24 05
adeline.clerge@normandie-univ.fr

Laboratoire SMS
Place Emile Blondel
76821 Mont-Saint-Aignan CEDEX
France

PhD Thesis

PH.D.: LIFE AND HEALTH SCIENCES – ABTE laboratory, Caen, France - October 2016 to December 2019

  • Title: HAP oxydés : étude des effets génotoxiques in-vitro et caractérisation des adduits à l’ADN par spectrométrie de masse.

Professional development in:    - Cytotoxicity and mutagenicity studies of oxy-PAHs

                                                        - Exposure on naked DNA and BEAS-2B cells

                                                        - Formation and characterization of adducts in UHPLC-MS/MS

  • Supervisor: Pr. Raphaël Delépée
  • Type of funding: Bourse ministérielle
  • Date of defense: December 19, 2019
Education
  • MASTER DEGREE of organic chemistry and spectrochemistry, University of Rouen, 2014 - 2016
  • BACHELOR DEGREE in chemistry, University of Rouen, 2011 – 2014
Work Experience

Research contract:

  • POSTDOCTORAL RESEARCHER: SMS laboratory, Mont Saint Aignan, 2019 (1 year)

Analysis of ionic biomolecules by ionic exchange chromatography hyphenated to mass spectrometry.

   - Optimization of coupling and detection in IC-MS/MS

   - Dosage of small organic acids involved in energy cycles.

  • MASTER 2 INTERNSHIP: SMS laboratory, Mont Saint Aignan, 2016 (6 months).

Development of stationary phases based on calix[6]arenes derivatives for liquid chromatography: synthesis, characterization and study of chromatographic performances by LSER.

  • LABORATORY TECHNICIAN, SMS laboratory & Interchim, 2016 (1 month).

   - Development of a dosage procedure for IC-MS/MS.

   - Scientific collaboration on the grafting of innovative stationary phases on core-shell silica.

  • MASTER 1 INTERNSHIP: SMS laboratory, Mont Saint Aignan, 2015 (5 weeks)

Evaluation of column in HILIC mode in HPLC: study of separation mechanisms according to analytical conditions on core-shell type columns.

  • LABORATORY TECHNICIAN, SMS laboratory, 2015 (1 month)

  - Separation and quantification of phenanthrene impurities: analysis in HPLC by fluorimetric detection.

  - Study of the preferential enrichment of tryptophan: determination of tryptophan solubility in different solvents; microscopic study of the crystals.

  • BACHELOR INTERNSHIP: Technologic university, Brno, Czech Republic, 2014 (3 months).

Multistep synthesis of polyfunctionalized thiophenes for optical and electronic applications: organic synthesis, purification on column and structural analysis.

 

Teaching in the department of Health: University of Caen, 2018-2019

  • Practical work of analytical chemistry – 2nd year of pharmacy studies – 96 hours
  • Practical work of clinical toxicology – 4th year of pharmacy studies – 30 hours
  • Practical work of galenic and analytical development – 4th year of pharmacy studies, industry and research pathways – 4 hours.
Papers
  • Adeline Clergé, Jérémie Le Goff, Claire Lopez-Piffet, Sonnich Meier, Stéphanie Lagadu, Isabelle Vaudorne, Victor Babin, Thomas Cailly, Raphaël Delépée, 2020. Investigation by mass spectrometry and 32P post-labelling of DNA adducts formation from 1,2-naphthoquinone, an oxydated metabolite of naphthalene. Chemosphere. 263.
  • Adeline Clergé, Jérémie Le Goff, Claire Lopez, Jérôme Ledauphin, Raphaël Delépée, 2019. Oxy-PAHs: occurrence in the environment and potential genotoxic/mutagenic risk assessment for human health. Crit. Rev. Toxicol. 49 (Sep).
Awards
  • BEST POSTER AWARD:

Study of the genotoxicity of 7 oxy-PAHs: focus on DNA adducts; A. Clergé, J. Le Goff, E. Brotin, I. Vaudorne, S. Lagadu, R. Delépée - EEMGS, 19 to 22 May 2019, Rennes;

Symposium Participation

ORAL PRESENTATIONS:

  • Synthesis and characterization by UHPLC-MS/MS of DNA adducts from 4 oxy-PAHs for the evaluation of their genotoxicity; A. Clergé, J. Le Goff, I. Vaudorne, S. Lagadu, R. Delépée - HPLC 2019, 16 au 20 juin 2019, Milan.
  • Stratégie pour le criblage de l’exposition humaine à des composés génotoxiques ; A. Clergé, J Le Goff, I. Vaudorne, S. Lagadu, R. Delépée - Club Chromatographie Val-de-Seine, 04 juin 2019, Deauville.
  • Evaluation de la génotoxicité du naphtalène par la formation d’adduits entre la 1,2-naphthoquinone et l’ADN ; A. Clergé, J. Le Goff, R. Delépée - Colloque SFTG-SPTC, 01 et 02 octobre 2018, Paris.
  • Caractérisation par UHPLC-MS/MS des profils d’adduit à l’ADN pour l’évaluation de la génotoxicité des polluants aériens ; A. Clergé, J. Le Goff, I. Vaudorne, S. Lagadu, R. Delépée - Club Chromatographie Val-de-Seine, 23 novembre 2017, Caen.
  • Cytotoxicité et génotoxicité des oxy-HAP pour les cellules pulmonaires humaines BEAS-2B ; A. Clergé, J. Le Goff, R. Delépée - Journées scientifiques du Club Jeunes de l’AFSEP, 13 et 14 novembre 2017, Montpellier.

 

POSTERS - FLASHPOSTERS:

  • Study of the genotoxicity of 7 oxy-PAHs: focus on DNA adducts; A. Clergé, J. Le Goff, E. Brotin, I. Vaudorne, S. Lagadu, R. Delépée - EEMGS, 19 au 22 mai 2019, Rennes.
  • Synthèse et caractérisation par UHPLC-MS/MS d’adduits entre l’ADN et 7 oxy-HAP pour l’évaluation de leur génotoxicité; Flashposter ; A. Clergé, J. Le Goff, I. Vaudorne, S. Lagadu, R. Delépée - SEP 2019, 25 au 28 mars 2019, Paris.
  • Synthesis, characterization and quantification by UHPLC-MS/MS of DNA adducts from 5 oxy-PAHs for the evaluation of their genotoxicity; A. Clergé, J. Le Goff, E. Brotin, I. Vaudorne, S. Lagadu, R. Delépée - ISC 2018, 23 au 27 septembre 2018, Cannes.
  • Evaluation of genotoxicity and mutagenicity of 1,2-naphthoquinone; A. Clergé, J. Le Goff, I. Vaudorne, S. Lagadu, R. Delépée - Journée Normande de Recherche Biomédicale, 20 septembre 2018, Rouen.
  • Evidences of genotoxic properties of oxy-PAHs and aldehydes using DNA adducts as a contribution to cancer etiology comprehension in exposome context; A. Clergé and H. Alamil, M. Lechevrel, J. Le Gofd, Z. Dagher, R. Delépée - Journée Normande de Recherche Biomédicale, 14 novembre 2017, Caen.
  • Nouvelle stratégie concernant l’hydrolyse de l’ADN pour l’analyse des adduits dans le contexte de l’exposome ; A. Clergé, J. Le Goff, R. Delépée - SEP 2017, 28 au 30 mars 2017, Paris.
Scientific Skills
Organic Synthesis assisted by microwaves
Conception of stationary phases for HPLC
Development of chromatographic methods for HPLC
Development of chromatographic methods coupled with mass spectrometry
Statistic treatment of data
Spectroscopy (Raman, Infra-rouge, UV-vis, fluorescence)
NMR spectroscopy
Solid-state characterization (polymorphism, solvates, cocrystals, solid-solid transition)