Mrs CLERGE Adeline 12/05/1991 02 35 52 24 05 |
Laboratoire SMS |
PH.D.: LIFE AND HEALTH SCIENCES – ABTE laboratory, Caen, France - October 2016 to December 2019
- Title: HAP oxydés : étude des effets génotoxiques in-vitro et caractérisation des adduits à l’ADN par spectrométrie de masse.
Professional development in: - Cytotoxicity and mutagenicity studies of oxy-PAHs
- Exposure on naked DNA and BEAS-2B cells
- Formation and characterization of adducts in UHPLC-MS/MS
- Supervisor: Pr. Raphaël Delépée
- Type of funding: Bourse ministérielle
- Date of defense: December 19, 2019
- MASTER DEGREE of organic chemistry and spectrochemistry, University of Rouen, 2014 - 2016
- BACHELOR DEGREE in chemistry, University of Rouen, 2011 – 2014
Research contract:
- POSTDOCTORAL RESEARCHER: SMS laboratory, Mont Saint Aignan, 2019 (1 year)
Analysis of ionic biomolecules by ionic exchange chromatography hyphenated to mass spectrometry.
- Optimization of coupling and detection in IC-MS/MS
- Dosage of small organic acids involved in energy cycles.
- MASTER 2 INTERNSHIP: SMS laboratory, Mont Saint Aignan, 2016 (6 months).
Development of stationary phases based on calix[6]arenes derivatives for liquid chromatography: synthesis, characterization and study of chromatographic performances by LSER.
- LABORATORY TECHNICIAN, SMS laboratory & Interchim, 2016 (1 month).
- Development of a dosage procedure for IC-MS/MS.
- Scientific collaboration on the grafting of innovative stationary phases on core-shell silica.
- MASTER 1 INTERNSHIP: SMS laboratory, Mont Saint Aignan, 2015 (5 weeks)
Evaluation of column in HILIC mode in HPLC: study of separation mechanisms according to analytical conditions on core-shell type columns.
- LABORATORY TECHNICIAN, SMS laboratory, 2015 (1 month)
- Separation and quantification of phenanthrene impurities: analysis in HPLC by fluorimetric detection.
- Study of the preferential enrichment of tryptophan: determination of tryptophan solubility in different solvents; microscopic study of the crystals.
- BACHELOR INTERNSHIP: Technologic university, Brno, Czech Republic, 2014 (3 months).
Multistep synthesis of polyfunctionalized thiophenes for optical and electronic applications: organic synthesis, purification on column and structural analysis.
Teaching in the department of Health: University of Caen, 2018-2019
- Practical work of analytical chemistry – 2nd year of pharmacy studies – 96 hours
- Practical work of clinical toxicology – 4th year of pharmacy studies – 30 hours
- Practical work of galenic and analytical development – 4th year of pharmacy studies, industry and research pathways – 4 hours.
- Adeline Clergé, Jérémie Le Goff, Claire Lopez-Piffet, Sonnich Meier, Stéphanie Lagadu, Isabelle Vaudorne, Victor Babin, Thomas Cailly, Raphaël Delépée, 2020. Investigation by mass spectrometry and 32P post-labelling of DNA adducts formation from 1,2-naphthoquinone, an oxydated metabolite of naphthalene. Chemosphere. 263.
- Adeline Clergé, Jérémie Le Goff, Claire Lopez, Jérôme Ledauphin, Raphaël Delépée, 2019. Oxy-PAHs: occurrence in the environment and potential genotoxic/mutagenic risk assessment for human health. Crit. Rev. Toxicol. 49 (Sep).
- BEST POSTER AWARD:
Study of the genotoxicity of 7 oxy-PAHs: focus on DNA adducts; A. Clergé, J. Le Goff, E. Brotin, I. Vaudorne, S. Lagadu, R. Delépée - EEMGS, 19 to 22 May 2019, Rennes;
ORAL PRESENTATIONS:
- Synthesis and characterization by UHPLC-MS/MS of DNA adducts from 4 oxy-PAHs for the evaluation of their genotoxicity; A. Clergé, J. Le Goff, I. Vaudorne, S. Lagadu, R. Delépée - HPLC 2019, 16 au 20 juin 2019, Milan.
- Stratégie pour le criblage de l’exposition humaine à des composés génotoxiques ; A. Clergé, J Le Goff, I. Vaudorne, S. Lagadu, R. Delépée - Club Chromatographie Val-de-Seine, 04 juin 2019, Deauville.
- Evaluation de la génotoxicité du naphtalène par la formation d’adduits entre la 1,2-naphthoquinone et l’ADN ; A. Clergé, J. Le Goff, R. Delépée - Colloque SFTG-SPTC, 01 et 02 octobre 2018, Paris.
- Caractérisation par UHPLC-MS/MS des profils d’adduit à l’ADN pour l’évaluation de la génotoxicité des polluants aériens ; A. Clergé, J. Le Goff, I. Vaudorne, S. Lagadu, R. Delépée - Club Chromatographie Val-de-Seine, 23 novembre 2017, Caen.
- Cytotoxicité et génotoxicité des oxy-HAP pour les cellules pulmonaires humaines BEAS-2B ; A. Clergé, J. Le Goff, R. Delépée - Journées scientifiques du Club Jeunes de l’AFSEP, 13 et 14 novembre 2017, Montpellier.
POSTERS - FLASHPOSTERS:
- Study of the genotoxicity of 7 oxy-PAHs: focus on DNA adducts; A. Clergé, J. Le Goff, E. Brotin, I. Vaudorne, S. Lagadu, R. Delépée - EEMGS, 19 au 22 mai 2019, Rennes.
- Synthèse et caractérisation par UHPLC-MS/MS d’adduits entre l’ADN et 7 oxy-HAP pour l’évaluation de leur génotoxicité; Flashposter ; A. Clergé, J. Le Goff, I. Vaudorne, S. Lagadu, R. Delépée - SEP 2019, 25 au 28 mars 2019, Paris.
- Synthesis, characterization and quantification by UHPLC-MS/MS of DNA adducts from 5 oxy-PAHs for the evaluation of their genotoxicity; A. Clergé, J. Le Goff, E. Brotin, I. Vaudorne, S. Lagadu, R. Delépée - ISC 2018, 23 au 27 septembre 2018, Cannes.
- Evaluation of genotoxicity and mutagenicity of 1,2-naphthoquinone; A. Clergé, J. Le Goff, I. Vaudorne, S. Lagadu, R. Delépée - Journée Normande de Recherche Biomédicale, 20 septembre 2018, Rouen.
- Evidences of genotoxic properties of oxy-PAHs and aldehydes using DNA adducts as a contribution to cancer etiology comprehension in exposome context; A. Clergé and H. Alamil, M. Lechevrel, J. Le Gofd, Z. Dagher, R. Delépée - Journée Normande de Recherche Biomédicale, 14 novembre 2017, Caen.
- Nouvelle stratégie concernant l’hydrolyse de l’ADN pour l’analyse des adduits dans le contexte de l’exposome ; A. Clergé, J. Le Goff, R. Delépée - SEP 2017, 28 au 30 mars 2017, Paris.